A study of normal and malignant thymus tissue of the teleost Astyanax mexicanus in tissue culture. Bulletin of the AMNH ; v. 124, article 3

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New York : [American Museum of Natural History]
"One. Normal and malignant thymus tissues of the adult teleost Astyanax mexicanus were successfully grown in tissue culture for the first time, with the use of the double cover-slip technique of Maximow. 2. Migration of thymocytes and thymoblasts was observed within the first few hours of in-vitro growth in the normal thymus. Other free cells that also made an early appearance included erythrocytes, small eosinophilic wandering cells, macrophages, and melanophores. Proliferation of epithelial reticular cells was usually observed between 36 and 48 hours after explantation, although occasionally some cells were seen as early as 24 hours. Multinucleation in the epithelial cells is described and discussed. Fibroblasts, present only in cultures of older fish, were the last cells to proliferate, usually after 48 hours. A description of these various cell types and their modes of migration or proliferation is presented. Comparisons are made between the normal thymus in vivo (through histological studies) and the thymus in vitro. 3. Histochemical determinations revealed that the epithelial cytoplasmic inclusions were predominantly lipid in nature (neutral fats and phospholipids). Some of the inclusions contained acid mucopolysaccharides. The possible significance of these results is discussed. 4. There were no clear-cut determinable effects of either mammalian cortisone or whole adrenal extract, in the dosages used, on the normal thymus in vitro. The lack of a valid criterion for such determinations in tissue cultures of thymocytes is discussed. 5. A description is given of the morphology in tissue culture of two spontaneous lymphosarcomas (reputedly of thymic origin), which occurred in our laboratory, in Astyanax. Comparison is made with normal thymus tissue. In general, the rate and extent of proliferation of cells in the tumor cultures far exceeded those in normal thymus cultures. With the limited data available, no valid conclusions can be drawn concerning the effects of various mammalian compounds (compound E, compound A, whole adrenal extract, and ACTH), on the lymphosarcoma in vitro"--P. 96.
p. 73-100, [13] p. of plates : ill. ; 27 cm.
Abridgment of thesis (Ph. D.)--New York University.
Includes bibliographical references (p. 96-100).