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A study of normal and malignant thymus tissue of the teleost Astyanax mexicanus in tissue culture. Bulletin of the AMNH ; v. 124, article 3

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dc.contributor.author Friedman, Libby Rosenbloom. en_US
dc.date.accessioned 2005-10-06T15:54:20Z
dc.date.available 2005-10-06T15:54:20Z
dc.date.issued 1962 en_US
dc.identifier.uri http://hdl.handle.net/2246/1978
dc.description p. 73-100, [13] p. of plates : ill. ; 27 cm. en_US
dc.description Abridgment of thesis (Ph. D.)--New York University. en_US
dc.description Includes bibliographical references (p. 96-100). en_US
dc.description.abstract "One. Normal and malignant thymus tissues of the adult teleost Astyanax mexicanus were successfully grown in tissue culture for the first time, with the use of the double cover-slip technique of Maximow. 2. Migration of thymocytes and thymoblasts was observed within the first few hours of in-vitro growth in the normal thymus. Other free cells that also made an early appearance included erythrocytes, small eosinophilic wandering cells, macrophages, and melanophores. Proliferation of epithelial reticular cells was usually observed between 36 and 48 hours after explantation, although occasionally some cells were seen as early as 24 hours. Multinucleation in the epithelial cells is described and discussed. Fibroblasts, present only in cultures of older fish, were the last cells to proliferate, usually after 48 hours. A description of these various cell types and their modes of migration or proliferation is presented. Comparisons are made between the normal thymus in vivo (through histological studies) and the thymus in vitro. 3. Histochemical determinations revealed that the epithelial cytoplasmic inclusions were predominantly lipid in nature (neutral fats and phospholipids). Some of the inclusions contained acid mucopolysaccharides. The possible significance of these results is discussed. 4. There were no clear-cut determinable effects of either mammalian cortisone or whole adrenal extract, in the dosages used, on the normal thymus in vitro. The lack of a valid criterion for such determinations in tissue cultures of thymocytes is discussed. 5. A description is given of the morphology in tissue culture of two spontaneous lymphosarcomas (reputedly of thymic origin), which occurred in our laboratory, in Astyanax. Comparison is made with normal thymus tissue. In general, the rate and extent of proliferation of cells in the tumor cultures far exceeded those in normal thymus cultures. With the limited data available, no valid conclusions can be drawn concerning the effects of various mammalian compounds (compound E, compound A, whole adrenal extract, and ACTH), on the lymphosarcoma in vitro"--P. 96. en_US
dc.format.extent 25660344 bytes
dc.format.mimetype application/pdf
dc.language eng en_US
dc.language.iso en_US
dc.publisher New York : [American Museum of Natural History] en_US
dc.relation.ispartofseries Bulletin of the American Museum of Natural History ; v. 124, article 3 en_US
dc.subject.lcc QH1 .A4 vol.124, art.3, 1962 en_US
dc.subject.lcsh Astyanax mexicanus -- Histopathology. en_US
dc.subject.lcsh Thymus. en_US
dc.subject.lcsh Thymus -- Tumors. en_US
dc.subject.lcsh Tissue culture. en_US
dc.title A study of normal and malignant thymus tissue of the teleost Astyanax mexicanus in tissue culture. Bulletin of the AMNH ; v. 124, article 3 en_US
dc.title.alternative Thymus tissue en_US
dc.type text en_US


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  • Bulletin of the American Museum of Natural History
    The Bulletin, published continuously since 1881, consists of longer monographic volumes in the field of natural sciences relating to zoology, paleontology, and geology. Current numbers are published at irregular intervals. The Bulletin was originally a place to publish short papers, while longer works appeared in the Memoirs. However, in the 1920s, the Memoirs ceased and the Bulletin series began publishing longer papers. A new series, the Novitates , published short papers describing new forms.

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